Rapid changes in transcriptomic profile and mitochondrial function in human soleus muscle after 3-day dry immersion

Rapid changes in transcriptomic profile and mitochondrial function in human soleus muscle after 3-day dry immersion Full article

Journal

Journal of Applied Physiology
ISSN: 1522-1601 , E-ISSN: 8750-7587

Output data

Year: 2023, Volume: 134, Number: 5, Pages: 1256-1264 Pages count : 9 DOI: 10.1152/japplphysiol.00048.2023

Authors

Popov Daniil V ^1,2 , Makhnovskii Pavel A ¹ , Zgoda Viktor G ³ , Gazizova Guzel R ⁴ , Vepkhvadze Tatiana F ^1,2 , Lednev Egor M ¹ , Motanova Evgeniia S ^1,5 , Lysenko Evgeny A ^1,6 , Orlov Oleg I ¹ , Tomilovskaya Elena S ¹

Affiliations

1	Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia
2	Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University, Moscow, Russia
3	V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia
4	Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Russia
5	Department of Biomedical Sciences, University of Padua, Padua, Italy
6	Department of Biology, University of Padua, Padua, Italy

We aimed to explore the effect of the 3-day dry immersion, a model of physical unloading, on mitochondrial function, transcriptomic and proteomic profiles in a slow-twitch soleus muscle of six healthy females. We registered that a marked reduction (25– 34%) in the ADP-stimulated respiration in permeabilized muscle fibers was not accompanied by a decrease in the content of mitochondrial enzymes (mass spectrometry-based quantitative proteomics), hence, it is related to the disruption in regulation of respiration. We detected a widespread change in the transcriptomic profile (RNA-seq) upon dry immersion. Downregulated mRNAs were strongly associated with mitochondrial function, as well as with lipid metabolism, glycolysis, insulin signaling, and various transporters. Despite the substantial transcriptomic response, we found no effect on the content of highly abundant proteins (sarcomeric, mitochondrial, chaperon, and extracellular matrix-related, etc.) that may be explained by long half-life of these proteins. We suggest that during short-term disuse the content of some regulatory (and usually low abundant) proteins such as cytokines, receptors, transporters, and transcription regulators is largely determined by their mRNA concentration. These mRNAs revealed in our work may serve as putative targets for future studies aimed at developing approaches for the prevention of muscle deconditioning induced by disuse.

Popov D.V. , Makhnovskii P.A. , Zgoda V.G. , Gazizova G.R. , Vepkhvadze T.F. , Lednev E.M. , Motanova E.S. , Lysenko E.A. , Orlov O.I. , Tomilovskaya E.S.
Rapid changes in transcriptomic profile and mitochondrial function in human soleus muscle after 3-day dry immersion
Journal of Applied Physiology. 2023. V.134. N5. P.1256-1264. DOI: 10.1152/japplphysiol.00048.2023 WOS Scopus РИНЦ PMID OpenAlex

Published online:

Apr 13, 2023

Web of science:	WOS:000993080200002
Scopus:	2-s2.0-85159542152
Elibrary:	62183174
PMID:	37055032
OpenAlex:	W4365457644

DB	Citing
OpenAlex	12
Scopus	6