Microscopy and image analysis of cell-derived decellularized extracelluler matrix

Microscopy and image analysis of cell-derived decellularized extracelluler matrix Full article

Journal

Cell and Tissue Biology
ISSN: 1990-5203 , E-ISSN: 1990-519X

Output data

Year: 2025, Volume: 19, Number: 1, Pages: 33-47 Pages count : 15 DOI: 10.1134/S1990519X25010043

Tags

cultured cells, extracellular matrix, decellularization, microscopy, morphometry

Authors

Melik-Pashaev Aleksei Érnestovich ^1,2 , Matveeva Diana Konstantinovna ¹ , Buravkov Sergei Valentinovich ^1,2 , Atyakshin D.A. ^3,4 , Kochetova Évelina Sergeevna ^1,2 , Andreeva Elena Romualʹdovna ¹

Affiliations

1	IBMP RAS
2	Lomonosov Moscow State University
3	Peoples' Friendship University of Russia named after Patrice Lumumba
4	Research Institute of Experimental Biology and Medicine, Burdenko Voronezh State Medical University

Cell-derived decellularized extracellular matrix (dcECM) attracts increasing research interest as a key regulator of the local microenvironment. Furthermore, the simplicity of manufacturing, tissue specificity and lack of limitations inherent to tissue decellularized extracellular matrix determine the feasibility of its use within the realm of regenerative medicine and tissue engineering. The present review seeks to systematize the current approaches employed in dcECM microscopy, with particular emphasis on the possibility of the observations of vital native ECM structures. Current methods of morphometry of cellular dcECM are also considered. The authors present their own data obtained during the study of dcECM of cultured human multipotent mesenchymal stromal cells (MSCs) as illustrations.

Melik-Pashaev A.É. , Matveeva D.K. , Buravkov S.V. , Atyakshin D.A. , Kochetova É.S. , Andreeva E.R.
Microscopy and image analysis of cell-derived decellularized extracelluler matrix
Cell and Tissue Biology. 2025. V.19. N1. P.33-47. DOI: 10.1134/S1990519X25010043 OpenAlex

Submitted:	Aug 7, 2024
Accepted:	Aug 8, 2024
Published print:	Jan 20, 2025

OpenAlex:

W4406632397

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