Abstract: Cell technologies have provided promising tools for modulating the properties of multipo-tent mesenchymal stem/stromal cells (MSCs) to meet the needs of cell therapy as well as tissue engineering and regenerative medicine (TERM). The ex vivo preconditioning is di-rected at enhancing the engraftment of MSCs and activating their secretory activity, pri-marily the production of soluble mediators. The present review aims to highlight the un-derestimated effect of the most accepted preconditioning approaches on the modification of the important set of insoluble molecules secreted by MSCs into extracellular space - the extracellular matrix (ECM). A thorough review of the published literature was performed, with particular emphasis on ECM-related data. The analysis of data on ECM changes showed that most of the applied preconditioning methods: hypoxia, inflammatory prim-ing, pharmacological agents, 3D culture and scaffolds - generally stimulate ECM produc-tion, increase the deposition of growth factors, promote alignment, and increase ECM stiffness. There are already preliminary results demonstrating the successful application of preconditioned ECM for promoting angiogenesis, targeted stromal lineage differentia-tion, and other therapeutic goals. The prospects for further research in this area are dis-cussed

Cite: Andreeva E.R. , Zhidkova O.V. , Matveeva D.K. , Gornostaeva A.N. , Lobanova M.V. , Buravkova L.B.
Ex Vivo Preconditioning as a Useful Tool for Modification of the Extracellular Matrix of Multipotent Mesenchymal Stromal Cells
International Journal of Molecular Sciences. 2025. V.26. 6301 :1-34. DOI: 10.3390/ijms26136301

Dates:

Submitted:	Apr 29, 2025
Accepted:	Jun 27, 2025
Published print:	Jun 30, 2025
Published online:	Jun 30, 2025

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