Homotypical Regulation of the Functional Activity of Multipotent Mesenchymal Stromal Cells: The Role of Gap Junctions Full article
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Cell and Tissue Biology
ISSN: 1990-5203 , E-ISSN: 1990-519X |
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Output data | Year: 2023, Volume: 17, Number: 2, Pages: 122-132 Pages count : 11 DOI: 10.1134/s1990519x23020049 | ||
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Abstract:
Perivascular multipotent mesenchymal stromal cells (MSCs) are considered as most likely putative
depot for MSCs’ mobilization in the case of tissue damage. Here, we analyzed the involvement of direct
homotypic gap junctions (GJs) intercellular communication in realization of MSC functions requested in tissue healing and repair: the state of intracellular compartments; paracrine, in particular, angiogenic, activity,
migration and phagocytosis. GJIC in growth arrested MSCs was suppressed using a specific inhibitor, carbenoxolone. Flow cytometry showed a doubling in mitochondrial mass in MSCs with blocked GJs
(MitoTracker Green probe). The transmembrane mitochondrial potential (JC-1 probe) and the levels of reactive oxygen species (ROS) (measured using a CM-H2DCFDA probe), as well as the activity of the lysosomal
compartment (LysoTracker Green probe), did not change. MSCs with blocked GJs migrated less actively in
the wound healing assay. The phagocytosis of latex beads was reduced. According to multiplex immunofluorescence assay, the levels of the IL-6, IL-8, and MCP-3 pleiotropic cytokines were significantly lower in the
conditioned medium (CM) from MSCs with blocked GJs. This CM did not stimulate the non-targeted
migration of endothelial cells and the growth of the tubule complexes in the chorioallantois membrane of the
quail embryo. It has been suggested that a decrease in the efficacy of direct intercellular communication
between MSCs and MSCs in vivo may negatively affect both the functioning of stromal cell functions on site
as well as their mobilization from tissue depots
Cite:
Ezdakova M.I.
, Matveeva D.K.
, Andreeva E.R.
Homotypical Regulation of the Functional Activity of Multipotent Mesenchymal Stromal Cells: The Role of Gap Junctions
Cell and Tissue Biology. 2023. V.17. N2. P.122-132. DOI: 10.1134/s1990519x23020049 Scopus РИНЦ OpenAlex
Homotypical Regulation of the Functional Activity of Multipotent Mesenchymal Stromal Cells: The Role of Gap Junctions
Cell and Tissue Biology. 2023. V.17. N2. P.122-132. DOI: 10.1134/s1990519x23020049 Scopus РИНЦ OpenAlex
Identifiers:
Scopus: | 2-s2.0-85158017047 |
Elibrary: | 62062931 |
OpenAlex: | W4367325967 |
Citing:
DB | Citing |
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OpenAlex | 1 |