OXIDATIVE STRESS SIGNS IN BLOOD PROTEOME ANALYSIS OF FEMALE VOLUNTEERS IN FIVE-DAY DRY IMMERSION TEST

OXIDATIVE STRESS SIGNS IN BLOOD PROTEOME ANALYSIS OF FEMALE VOLUNTEERS IN FIVE-DAY DRY IMMERSION TEST Научная публикация

Журнал

Медицина экстремальных ситуаций
ISSN: 2306-6326 , E-ISSN: 2713-2773

Вых. Данные

Год: 2025, Том: 27, Номер: 2, Страницы: 205-212 Страниц : 8 DOI: 10.47183/mes.2025-251

Ключевые слова

dry immersion; proteome; chromatography-mass spectrometry; dry blood spots; oxidative stress

Авторы

Kashirina Daria N. ¹ , Pastushkova Ludmila Kh ¹ , Brzhozovskiy Alexander G. ^1,2 , Kononikhin Alexey S. ^1,2 , Nikolaev Evgeniy N. ² , Larina Irina M. ¹

Организации

1	Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia
2	Skolkovo Institute of Science and Technology, Moscow, Russia

Информация о финансировании (1)

Министерство науки и высшего образования Российской Федерации

FMFR-2024-0032

Introduction. Dry immersion is a model for reproducing the physiological effects of weightlessness. Such tests allow assessment of changes in the functions of the cardiovascular, musculoskeletal, and other body systems. Mass spectrometry instruments can be used for blood proteome analysis in order to establish mechanisms of physiological adaptation to spaceflight factors simulated in dry immersion tests. Objective. To clarify molecular participants in the acute period of adaptation of physiological systems to the conditions of simulated microgravity according to proteome analysis of dry blood spots of participants in a five-day dry immersion test. Materials and methods. The study involved eight healthy female volunteers (average age 30 ± 4.8 years). Blood proteins were analyzed using the method of dried blood spots; capillary blood dried on a special paper filter (Perkin Elmer) was used. The enzyme cleavage of proteins was performed using trypsin (Thermo Scientific, USA). Mixtures of tryptic peptides were analyzed by liquid chromatography-mass spectrometry (LC–MS) on a Dionex Ultimate 3000 nano-HPLC combined with a TimsTOF Pro mass spectrometer. Mass spectrometric analysis was performed using the method of parallel accumulation with sequential fragmentation (PASEF). The obtained LC–MS/MS data were semi-quantitatively analyzed using DIA-NN 1.8.1. Statistical analysis was performed in the Statistica 12 software package. Results. The molecular response to immersion conditions was found to lead to increased levels of antioxidant defense proteins, activation of catabolism processes and the pentose phosphate pathway. The levels of negative regulators of endopeptidases and iron homeostasis proteins decreased. The revealed elevated levels of NADPH oxidase activators indicate activation of NADPH oxidase under experimental conditions. These results may indicate the development of oxidative stress during immersion. Conclusions. The identified molecular participants in the female body’s response to immersion conditions can provide information about the signaling pathways and mechanisms involved in the response to hypokinesia, and in the future will contribute to the development of pharmacological measures to support the health of female astronauts. These results may also be useful for understanding the processes leading to adverse effects in people with low levels of physical activity.

Kashirina D.N. , Pastushkova L.K. , Brzhozovskiy A.G. , Kononikhin A.S. , Nikolaev E.N. , Larina I.M.
OXIDATIVE STRESS SIGNS IN BLOOD PROTEOME ANALYSIS OF FEMALE VOLUNTEERS IN FIVE-DAY DRY IMMERSION TEST
Медицина экстремальных ситуаций. 2025. V.27. N2. P.205-212. DOI: 10.47183/mes.2025-251 OpenAlex

Поступила в редакцию:	15 янв. 2025 г.
Принята к публикации:	28 апр. 2025 г.
Опубликована online:	4 июл. 2025 г.

OpenAlex:

W4411968133

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