Abstract: The aim of this study was to prevent initial changes in Drosophila melanogaster oocytes under simulated weightlessness and hypergravity at the 2 g level. Phospholipids with polyunsaturated fatty acids in the tail groups (essential phospholipids) at a concentration of 500 mg/kg of nutrient medium were used as a protective agent. Cell stiffness was determined using atomic force microscopy, the change in the oocytes’ area was assessed as a mark of deformation, and the contents of cholesterol and neutral lipids were determined using fluorescence microscopy. The results indicate that the administration of essential phospholipids leads to a decrease in the cholesterol content in the oocytes’ membranes by 13% (p < 0.05). The stiffness of oocytes from flies that received essential phospholipids was 14% higher (p < 0.05) and did not change during 6 h of simulated weightlessness or hypergravity, and neither did the area, which indicates their resistance to deformation. Moreover, the exposure to simulated weightlessness and hypergravity of oocytes from flies that received a standard nutrient medium led to a more intense loss of cholesterol from cell membranes after 30 min by 13% and 18% (p < 0.05), respectively, compared to the control, but essential phospholipids prevented this effect.

Cite: Gogichaeva K.K. , Ogneva I.V.
Administration of Essential Phospholipids Prevents Drosophila Melanogaster Oocytes from Responding to Change in Gravity
Cells. 2024. V.13. N18. 1593 . DOI: 10.3390/cells13181593 WOS Scopus РИНЦ PMID OpenAlex

Dates:

Submitted:	Aug 28, 2024
Accepted:	Sep 21, 2024
Published online:	Sep 22, 2024

Identifiers:

Web of science:	WOS:001326466500001
Scopus:	2-s2.0-85205151943
Elibrary:	74104804
PMID:	39329774
OpenAlex:	W4402750812

Citing:

DB	Citing
OpenAlex	1

Altmetrics: