Реферат: The aim of this study was to prevent initial changes in Drosophila melanogaster oocytes under simulated weightlessness and hypergravity at the 2 g level. Phospholipids with polyunsaturated fatty acids in the tail groups (essential phospholipids) at a concentration of 500 mg/kg of nutrient medium were used as a protective agent. Cell stiffness was determined using atomic force microscopy, the change in the oocytes’ area was assessed as a mark of deformation, and the contents of cholesterol and neutral lipids were determined using fluorescence microscopy. The results indicate that the administration of essential phospholipids leads to a decrease in the cholesterol content in the oocytes’ membranes by 13% (p < 0.05). The stiffness of oocytes from flies that received essential phospholipids was 14% higher (p < 0.05) and did not change during 6 h of simulated weightlessness or hypergravity, and neither did the area, which indicates their resistance to deformation. Moreover, the exposure to simulated weightlessness and hypergravity of oocytes from flies that received a standard nutrient medium led to a more intense loss of cholesterol from cell membranes after 30 min by 13% and 18% (p < 0.05), respectively, compared to the control, but essential phospholipids prevented this effect.

Библиографическая ссылка: Gogichaeva K.K. , Ogneva I.V.
Administration of Essential Phospholipids Prevents Drosophila Melanogaster Oocytes from Responding to Change in Gravity
Cells. 2024. V.13. N18. 1593 . DOI: 10.3390/cells13181593 WOS Scopus РИНЦ PMID OpenAlex

Даты:

Поступила в редакцию:	28 авг. 2024 г.
Принята к публикации:	21 сент. 2024 г.
Опубликована online:	22 сент. 2024 г.

Идентификаторы БД:

Web of science:	WOS:001326466500001
Scopus:	2-s2.0-85205151943
РИНЦ:	74104804
PMID (PubMed):	39329774
OpenAlex:	W4402750812

Цитирование в БД:

БД	Цитирований
OpenAlex	1

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